FIGURE 2.

All eIF4G isoforms are required to support Luc and Ω-LUC transcript levels. qPCR was performed for expression from 35S::Luc and 35S::Ω-Luc transgenes in 8-day-old WT seedlings in WT plants and in eifiso4e, eif4g, eifiso4g1, eifiso4g2, and eifiso4g1/2 mutants into which each transgene was crossed. In A, expression is reported relative to the level of Luc (white bars) and Ω-Luc (black bars) mRNAs in WT seedlings, which is set at a value of 1. In B, the transcript level of Ω-Luc (black bars) is reported relative to the Luc (white bars) transcript level (set at a value of 1) for WT and mutant seedlings.