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. 2015 Dec 7;9:112–122. doi: 10.2174/1874613601509010112

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© Laderoute et al.; Licensee Bentham Open.

This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Fig. (2) — Expression of HERV-K102 Env with vacuolation. (A) By immunohistology performed with the affinity purified ML4 rabbit antibody to HML-2 type 1 Env and with the affinity purified ML5 rabbit antibody to HERV-K102 Env diluted 1/1000 [3]. Expression is detected only in vacuolating cells, and only on CB cultured for 7 days but not in uncultured day 0 CB. Purified positive control rabbit antilymphocyte globulin (ALG) and negative control rabbit globulin (C) are also shown. (B) Western blotting with affinity purified ML4 antibody diluted 1/1000 on ML4 and ML5 immunoprecipitated envelope proteins (precursor 90 kDa, processed 55 kDa) from day 7 cultured CB cells but not isolated from uncultured day 0 CB cells. C refers to control purified rabbit antibody (pre-serum).