Fig. 5.

Tissue expression analysis of the EPO in the three schizothoracine fishes. a Six tissues (heart, spleen, liver, brain, muscle and gill) from three schizothoracine fish (G. dobula (Gd), S. nukiangensis Tsao (Snt) and S. prenanti (Sp)) were subjected to transcriptome sequencing. EPO expression levels were calculated based on normalized expression values. The gene expression levels of EPO in G. dobula were derived from three replicates. To compare EPO gene expression levels between the highly specialized and the non-highly specialized schizothoracines, S. nukiangensis Tsao and S. prenanti were set as the non-highly specialized schizothoracine fish group. The statistical significance was determined using a one-tailed unpaired Student’s t-test with P < 0.05. b Normalized expression values of the EPO genes in 6 tissues from three schizothoracine fish (Gd, Snt and Sp) and the relevant statistical analyses. P value ^a and P value ^b were the P values adjusted by FDR (false discovery rate) and Bonferroni correction, respectively