Fig. 4.
SERV-K1 Env-specific antibodies are common in rhesus macaques. a Overlapping 15-mer peptides spanning SIVmac239 Env were used in a peptide-based ELISA for detection of specific antibodies in the serum of r02120. b Overlapping 15-mer peptides spanning SERV-K1 Env were used in a peptide-based ELISA for detection of specific antibodies in the serum of 02120 (black) as well as the plasma of 3 additional SIV-infected (red) and 2 SIV-naïve RMs (blue). Arrow indicates SERV-K1 Env541–555 15-mer, which is an epitope targeted in HIV-infected patients. Peptides indicated with asterisks (*) are shown in part c. Graphs in a, b indicate the major subunits and variable loops (V1–V5) of the Env proteins. c ELISA detection of antibody responses to six frequently-targeted SERV-K1 15-mer peptides in plasma from 21 SIV-naive rhesus macaques (blue). SIV-infected rhesus macaques from part b are shown for reference. All graphs display the average of two duplicate wells, background subtracted