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. 2016 Jan 15;9:2. doi: 10.1186/s13072-016-0051-y

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© Urahama et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Mutational analysis of H3.5 and H3.3. a Non-denaturing 6 % PAGE analysis of the purified nucleosomes containing H3.5 and H3.3 mutants, stained with ethidium bromide. Lane 1 represents the naked DNA used in the nucleosome reconstitution. Nucleosome core particles are denoted by NCPs. b Histone compositions of the purified nucleosomes containing H3.3 and H3.5 mutants, analyzed by 18 % SDS-PAGE with Coomassie Brilliant Blue staining. c Salt resistance assays of the H3.5 mutant nucleosomes, and d the H3.3 mutant nucleosomes. Bands corresponding to nucleosomes are indicated as NCPs. Asterisks represent bands corresponding to non-nucleosomal DNA-histone complexes [26]