Table 3.
Summary of experimental data for the colon cancer cell lines examined
| CaCo2 | HCT116 | HT29 | SW480 | |
|---|---|---|---|---|
| CRY1 endogenous mRNA levela | ↑↑↑ | ↑↑ | ↑↑ | ↑ |
| CRY2 endogenous mRNA levela | ↑↑↑ | ↑↑ | ↑↑ | ↑ |
| CRY1 endogenous protein levelb | ↑ | ↑↑↑ | ↑ | ↑↑ |
| CRY2 endogenous protein levelb | ↑↑ | ↑↑↑ | ↑ | ↑ |
| CRY1 ectopic protein level | ↑ | ↑↑↑ | ↑ | ↑ |
| CRY2 ectopic protein level | ↑ | ↑↑↑ | ↑ | ↑ |
| TP53 status | mutated (Ø) | wild-type | mutated (GOF) | mutated (GOF) |
| p53 endogenous protein level | Ø | ↑ | ↑↑↑ | ↑↑↑ |
| p53 endogenous protein level upon CRY1 ectopic expression | Ø | ↑↑ | = | = |
| p53 endogenous protein level upon CRY2 ectopic expression | Ø | ↑↑ | ↓ | = |
| ARNTL endogenous mRNA levela | ↑ | ↑↑↑ | ↑ | ↑ |
| c-MYC endogenous mRNA levela | ↑ | ↑↑↑ | ↑↑↑ | ↑ |
| WEE endogenous mRNA levela | ↑ | ↑↑ | ↑↑↑ | ↑↑ |
| Cell pre-apoptosis upon CRY1 ectopic expression | = | = | ↓↓ | ↓↓ |
| Cell pre-apoptosis upon CRY2 ectopic expression | ↓ | = | ↓ | ↓↓ |
| Cell apoptosis upon CRY1 ectopic expression | = | = | ↓↓ | ↓↓ |
| Cell apoptosis upon CRY2 ectopic expression | ↓ | = | ↓ | ↓↓ |
| Cell viability upon CRY1 ectopic expression | = | ↑↑ | ↑↑ | ↑↑ |
| Cell viability upon CRY2 ectopic expression | ↑ | ↑↑ | ↑↑ | ↑↑ |
| Cell cycle changes upon CRY1 ectopic expression | S↑ G2/M= | S↑ G2/M= | S↑ G2/M↓ | S = G2/M↑ |
| Cell cycle changes upon CRY2 ectopic expression | S↑ G2/M= | S↑ G2/M= | S↑ G2/M↓ | S↑ G2/M= |
| Cell pre-apoptosis upon treatment with OXA | ↑↑↑ | ↑↑ | ↑↑ | ↑↑ |
| Cell pre-apoptosis upon treatment with 5FU | ↑↑↑ | = | ↑↑ | ↑↑ |
| Cell apoptosis upon treatment with OXA | ↑↑↑ | ↑↑ | ↑↑ | ↑↑ |
| Cell apoptosis upon treatment with 5FU | ↑↑↑ | = | ↑↑ | ↑↑ |
| Cell viability upon CRY1 ectopic expression + OXAc | ↓ | ↓ | = | ↓ |
| Cell viability upon CRY2 ectopic expression + OXAc | ↓ | = | = | ↓ |
| Cell viability upon CRY1 ectopic expression + 5FUc | = | ↑ | ↓ | ↑ |
| Cell viability upon CRY2 ectopic expression + 5FUc | = | ↑ | = | ↑ |
acells harvested 21 h after synchronization with serum shock; bcells harvested 22 h after synchronization with serum shock; Ø protein not detected, GOF gain of function; ccompared to mock transfected cells