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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Cell Metab. 2010 Oct 6;12(4):373–385. doi: 10.1016/j.cmet.2010.08.001

Fig. 6. The active-site cysteine of the Grx domain is essential for iron metabolism.

Fig. 6

Gal-GRX4 cells lacking (−) or expressing wild-type (WT) Grx4-Myc or the Grx4-Myc mutant proteins C37S, C171S or C171A under the control of the GRX4 (A) or tetO7 (C) promoter from vector pCM189 were cultivated in SD medium. After 40 h, tenfold serial dilutions were spotted onto SC medium with glycerol. The levels of Grx4-Myc, endogenous Grx4, and cytosolic Hsp70 were assessed by immunostaining. (B, D) After 64 h cells from A and C were analyzed for FET3 promoter activities, or aconitase and catalase enzyme activities.