TABLE 5.
Micro-CT and dynamic histomorphometry analyses of femurs of female mice treated with iPTH (80 μg/kg/d) from 4 to 8 wk of age
| Parameter | Control PBS | Control iPTH | P | Change (%) | DMP-GHRKO PBS | DMP-GHRKO iPTH | P | Change (%) |
|---|---|---|---|---|---|---|---|---|
| Sample size (n) | 8 | 9 | 9 | 10 | ||||
| Body weight (g) | 17.00 ± 1.09 | 17.96 ± 1.59 | 0.170 | 5.38 | 17.41 ± 1.81 | 16.56 ± 1.28 | 0.248 | 5.16 |
| Bone length (mm) | 8.36 ± 0.42 | 8.54 ± 0.28 | 0.309 | 2.12 | 8.42 ± 0.36 | 8.39 ± 0.17 | 0.804 | 0.38 |
| Cortical bone, femoral middiaphysis | ||||||||
| Tt.Ar (mm2) | 1.55 ± 0.11 | 1.68 ± 0.14 | 0.056 | 8.49 | 1.51 ± 0.17 | 1.48 ± 0.11 | 0.629 | 2.20 |
| Ct.Ar (mm2) | 0.61 ± 0.04 | 0.72 ± 0.06 | <0.01 | +16.93 | 0.60 ± 0.08 | 0.60 ± 0.09 | 0.965 | 0.28 |
| RCA (%) | 39.93 ± 2.27 | 42.94 ± 0.68 | <0.01 | +7.52 | 39.92 ± 2.25 | 40.77 ± 3.40 | 0.532 | 2.13 |
| Ct.Th (mm) | 0.14 ± 0.00 | 0.16 ± 0.00 | <0.01 | +12.95 | 0.14 ± 0.01 | 0.14 ± 0.01 | 0.833 | 1.06 |
| Ma.Ar (mm2) | 0.93 ± 0.09 | 0.96 ± 0.07 | 0.523 | 2.90 | 0.91 ± 0.10 | 0.87 ± 0.04 | 0.370 | 3.86 |
| Robustness (mm−1) | 0.11 ± 0.00 | 0.12 ± 0.00 | 0.044 | +8.12 | 0.11 ± 0.00 | 0.11 ± 0.00 | 0.356 | 3.33 |
| J0 (mm−4) | 0.252 ± 0.03 | 0.319 ± 0.06 | <0.01 | +26.40 | 0.244 ± 0.05 | 0.235 ± 0.04 | 0.73 | 3.46 |
| TMD (g/cc) | 1.677 ± 0.06 | 1.668 ± 0.07 | 0.79 | 0.55 | 1.710 ± 0.10 | 1.688 ± 0.06 | 0.59 | 1.25 |
| Trabecular bone,distal femur | ||||||||
| BV/TV (%) | 7.01 ± 1.75 | 13.03 ± 5.43 | <0.01 | +85.85 | 6.92 ± 1.25 | 8.31 ± 4.33 | 0.365 | 20.13 |
| Tb.Th, (mm) | 0.041 ± 0.00 | 0.049 ± 0.00 | <0.01 | +20.55 | 0.043 ± 0.00 | 0.049 ± 0.00 | <0.01 | +15.86 |
| Tb.N (mm−1) | 1.68 ± 0.34 | 2.62 ± 1.11 | 0.036 | +56.10 | 1.60 ± 0.22 | 1.66 ± 0.86 | 0.838 | 3.84 |
| Tb.Sp (mm) | 0.24 ± 0.03 | 0.24 ± 0.04 | 0.822 | 1.80 | 0.24 ± 0.01 | 0.27 ± 0.03 | 0.031 | +13.75 |
| BMD (g/cc) | 0.26 ± 0.02 | 0.34 ± 0.06 | <0.01 | +28.59 | 0.27 ± 0.02 | 0.28 ± 0.04 | 0.485 | 4.83 |
| Histomorphometry, trabecular bone, distal femur | ||||||||
| Sample size (n) | 4 | 4 | 7 | 7 | ||||
| Mineralized surface (%) | 32.38 ± 1.25 | 26.77 ± 6.65 | 0.15 | 17.32 | 28.03 ± 4.57 | 32.66 ± 7.38 | 0.18 | 16.5 |
| MAR (μm/d) | 2.33 ± 0.27 | 3.48 ± 0.60 | <0.01 | +49.21 | 2.58 ± 0.43 | 2.636 ± 0.53 | 0.85 | 1.83 |
| BFR/BV (μm/d/mm) | 44.63 ± 5.63 | 48.90 ± 10.08 | 0.48 | 9.56 | 41.09 ± 10.02 | 45.31 ± 14.17 | 0.53 | 10.26 |
| Osteoclast/BS (n/10 mm) | 7.4 ± 2.9 | 11.7 ± 4.88 | 0.18 | 173.61 | 16.9 ± 5.1 | 26.5 ± 3.05 | 0.01 | +266.37 |
| Osteoblast/BS (n/mm) | 37.87 ± 13.66 | 58.94 ± 7.54 | 0.44 | 155.60 | 21.39 ± 10.74 | 31.10 ± 4.80 | 0.01 | +145.39 |
Values are presented as means ± sd. Significance was set P < 0.05; Student’s t tests. BS, bone surface.