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. 2015 Oct 14;30(2):702–715. doi: 10.1096/fj.15-280271

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This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — PLC and PKC mediate store-operated phosphorylation of TRPC1 proteins. A) Coimmunoprecipitation (IP) of freshly isolated rabbit portal vein tissue lysates with anti-phosphorylated serine (pSer) and threonine (pThr) antibodies followed by Western blotting (WB) with an anti-TRPC1 antibody shows that constitutive TRPC1 phosphorylation was reduced by pretreatment with U73122 or GF109203X, but not by U73343 (left panel). Pretreatment with CPA (middle panel) or BAPTA-AM (right panel) increased phosphorylation of TRPC1, which were inhibited by coapplication of U73122 or GF109203X. B) Mean relative band intensities normalized to control bands of data (n = 3 different tissue lysate preparations). **P < 0.01 vs. control; ^##P < 0.01 vs. CPA or BAPTA-AM.