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. 2015 Oct 14;30(2):702–715. doi: 10.1096/fj.15-280271

Figure 3.

Figure 3.

Activation of TRPC1 SOCs is mediated by PLCβ1. A) Western blots and mean data confirm that 2 different PLCβ1 shRNA sequences (shRNA1 and shRNA2) reduced PLCβ1 expression (n = 3 primary cell culture preparations). **P < 0.01. B) Representative traces show that peak amplitude of store-operated whole-cell TRPC1 currents was greatly reduced following transduction of cells with PLCβ1 shRNA1 compared to scrambled shRNA sequences. In the presence of scrambled shRNA, store-operated whole-cell currents were inhibited by U73122. C) Mean I/V relationships show that PLCβ1 knockdown with shRNA1 and shRNA2 reduced store-operated TRPC1 currents (n = 6). D) Representative recordings and mean data show that BAPTA-AM-evoked TRPC1 SOC activities were reduced by both PLCβ1 shRNA1 and shRNA2 sequences compared to scrambled shRNA, but this did not affect channel activation by phorbol 12,13-dibutyrate (PDBu) (n = 7). ***P < 0.001.