Figure 5.

Store-operated PLC activity is mediated by PLCβ1. Representative images and mean data show that transduction of VSMCs with either PLCβ1 shRNA1 (A) or shRNA2 (B) sequences prevented BAPTA-AM (red) inducing translocation of GFP-PLCδ1-PH signals to the cytosol. In both these conditions, noradrenaline (green) was still able to induce translocation of GFP-PLCδ1-PH signals from the plasma membrane to the cytosol (n = 20 cells for each PLCβ1 shRNA sequence from 3 different primary cell culture preparations). ***P < 0.01.