Figure 6.

Store depletion evoked associations between TRPC1, Gαq and PLCβ1. A) Representative Western blots show that in unstimulated primary cultured rabbit portal vein VSMCs, TRPC1 did not associate with Gαq or PLCβ1. BAPTA-AM induced associations between TRPC1 and Gαq, and TRPC1 and PLCβ1, which were reduced by transduction of cells with either PLCβ1 shRNA1 or shRNA2 sequences. Primary cultured rabbit portal vein cell lysates initially immunoprecipitated (IP) with anti-TRPC1 antibodies were then Western blotted (WB) with anti-Gαq or anti-PLCβ antibodies. B) Mean data for relative band intensities of BAPTA-AM-evoked interactions between TRPC1 and Gαq or PLCβ1 (n = 3, different primary cell culture preparations). Scram., scrambled. *P < 0.05.