Figure 7.

Store depletion induced colocalizations between TRPC1, Gαq and PLCβ1 at the plasma membrane. A) Representative PLA images from individual VSMCs show that BAPTA-AM induced fluorescent signals (red), which related to interactions between TRPC1 and Gαq, and TRPC1 and PLCβ1 in the presence of scrambled shRNA. B, C) Transduction of cells with PLCβ1 shRNA1 (B) and shRNA2 (C) sequences reduced BAPTA-AM-induced fluorescent signals between TRPC1 and PLCβ1 but did not alter those signals produced between TRPC1 and Gαq. D) Mean data show that both PLCβ1 shRNA1 and shRNA2 sequences prevented BAPTA-AM-induced punta formation between TRPC1 and PLCβ1 (n = 20, cells from 3 different experiments). *P < 0.05.