Figure 7.

Intracellular insulin content is compromised in the absence of PDIA6. A) PDIA6-sufficient and -deficient INS1 cells were lysed and analyzed by RIA for the human intracellular insulin. Each measurement was normalized to the protein content. Data are means ± sd of 3 independent experiments. *P ≤ 0.05; **P ≤ 0.01; Student’s t test; shPDIA6 vs. the corresponding shCtrl condition. B) Immunostaining of total insulin in either PDIA6-sufficient or -deficient INS1 cells. C) Quantitation of total fluorescence intensity from cells in (B).