Hepatic GR regulates sexually distinct inflammatory gene expression. A) RNA counts of GR in male and female GRflox/flox and LGRKO mice. Data are RNA counts (means ± sem, n = 3 animals). B) Western blot analysis of GR protein expression in male and female GRflox/flox and LGRKO mice. C) Nanostring analysis of male and female GRflox/flox and LGRKO mice showing significantly altered genes in each comparison. Differences in gene expression that reached P < 0.05 were significant. D) Venn diagram of nanostring results overlaying male GRflox/flox to male LGRKO and female GRflox/flox to female LGRKO mice. E) RNA counts of male-specific GR genes dysregulated by GR ablation. Data are represented as RNA counts (means ± sem, n = 3 animals). F) RNA counts of female-specific GR genes dysregulated by GR ablation. Data are represented as RNA counts (means ± sem, n = 3 animals). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.