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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Toxicol Res (Camb). 2015 Mar 17;4(5):1143–1158. doi: 10.1039/c4tx00236a

Fig. 4. E-cadherin is an AHR/KLF6 target gene.

Fig. 4

E-cadherin is a tumor suppressor protein involved in cell-cell adhesion. Loss of E-cadherin expression (or function) correlates positively with cancer progression and metastasis. Mice were treated by oral gavage with vehicle (−) or 20 μg/kg TCDD (+) for 2 hours. Chromatin immunoprecipitations (ChIP) were performed on livers using antibodies against the indicated proteins and the E-cadherin promoter (−220 to +37) encompassing a NC-XRE, amplified by PCR. The data show TCDD-dependent recruitment of the AHR and KLF6 to the E-cadherin promoter. This sequence was independently shown to harbor a KLF6 binding site in 3 separate human cancer cell lines261.