Table 1.
Antibodies used for western blotting in the current study
| Antibody name
|
Origin
|
Concentration
|
Source
|
Primary/secondary
|
Monoclonal/polyclonal
|
|---|---|---|---|---|---|
| Anti phospho-AKT (Ser473), #4051 | Mouse | 1:1000 | Cell Signaling Technology, New England Biolabs, Hertfordshire, UK | Primary | Monoclonal |
| Anti-AKT (total), #9272 | Rabbit | 1:1000 | Cell Signaling Technology, New England Biolabs | Primary | Polyclonal |
| Anti-diphospho-ERK-1 and 2, #M8159 | Mouse | 1:5000 | Sigma, St. Louis, MO, USA | Primary | Monoclonal |
| Anti-ERK-1 and 2 (total), #M5670 | Rabbit | 1:5000 | Sigma | Primary | Polyclonal |
| Anti-phospho-p70 S6 kinase (Thr389), #9206 | Mouse | 1:1000 | Cell Signaling Technology, New England Biolabs | Primary | Monoclonal |
| Anti-p70S6 kinase (total), #9202 | Rabbit | 1:1000 | Cell Signaling Technology, New England Biolabs | Primary | Polyclonal |
| Anti-chromogranin A, #ab15160 | Rabbit | 1:100 | Abcam, Cambridge, UK | Primary | Polyclonal |
| Anti-beta actin antibody, #ab6276 | Mouse | 1:5000 | Abcam | Primary | Monoclonal |
| Anti-rabbit IRDye_800, #611-132-002 | Goat | 1:5000 | LI-COR Biosciences, Cambridge, UK | Secondary | Polyclonal |
| Anti-mouse IRDye_680, #926-32220 | Goat | 1:5000 | LI-COR Biosciences | Secondary | Polyclonal |