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. 2015 Nov 6;106(12):1693–1704. doi: 10.1111/cas.12831

Figure 2.

Figure 2

Phosphatase and C2 domains of unphosphorylated PTEN are essential for inhibition of transforming growth factor β (TGFβ)‐induced signaling pathways in H358 lung cancer cells. (a) Schematic diagram of PTEN deletion mutants. We established H358ON cells expressing Dox‐dependent GFP‐PTEN4A lacking the phosphatase (Pho) domain (G4A Δ phosphatase), GFP‐PTEN4A lacking the C2 domain (G4A Δ C2), and GFP‐PTEN4A containing the C2 domain only (G4A C2 only). A representative blot from three independent experiments is shown as G4A Δ phosphatase (b), G4A Δ C2 (c), and G4A C2 only (d). (e–j) Total and phosphorylated Akt308 (e–g) and FAK (h–j) after treatment with vehicle or TGFβ were analyzed by Western blotting. The ratio of phosphorylated protein to total protein (pA308/A and pF/F) is presented as the intensity level relative to that in H358ON cells treated with vehicle in the absence of Dox. Data shown represent the means ± SE. The experiment was repeated three times with similar results. *P < 0.05. NS, not significant.