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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: J Med Chem. 2015 Aug 28;58(17):6938–6959. doi: 10.1021/acs.jmedchem.5b00801

Figure 7.

Figure 7

Uptake of [3H]7 by FRα and FRβ. RT16 (FRα) and D4 (FRβ) CHO cells were washed with acid (pH 3.5) buffered saline to remove FR-bound folates and then incubated at pH 7.4 with 50 nM [3H]7 at 0 °C (“Surface”) or at 37 °C for 60 min (“Total”). An additional condition involved treatment with 50 nM [3H]7 at 37 °C for 60 min, followed by an acid buffer wash to remove the extracellular FR-bound [3H]7 fraction (“Intracellular”). Cellular proteins were solubilized with NaOH for determinations of cell-associated [3H]7 and proteins. Experimental details are provided in the Experimental Procedures. Results are presented as mean values plus/minus SEM from three experiments.