Fig 3.
The fold change in the gene expression of interleukin-1beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), cyclooxygenese-2 (PTGS2) and monocyte chemoattractant factor (MCP-1) in the RVLM after 90 days of E2 exposure are shown above. The fold change for each mRNA was calculated by the comparative CT method using 2−ΔΔCt. The Ct values of all the genes were normalized to β-actin and the Ct values from different groups were expressed relative to that in young intact sham rats. * denotes significant difference (p<0.05) from control (YS) group.