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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Environ Mol Mutagen. 2015 Sep 10;57(1):58–64. doi: 10.1002/em.21982

Figure 2.

Figure 2

hsa-miR-548s and hsa-miR-4480 suppress IL-17A gene expression in an allele specific manner. (A) Targeting prediction of hsa-miR-548s to the 3′UTR of IL-17A based on rs7747909 allele. The binding energy of the miRNA to the major G allele is stronger than the minor A allele. (B) Targeting prediction of hsa-miR-4480 to the 3′UTR of IL-17A based on rs7747909 allele. The binding energy of the miRNA to the major G allele is stronger than the minor A allele. (C) Luciferase reporter assay to investigate the effect of each allele of rs7747909 in ARPE-19 retinal pigmented epithelium cells. ARPE-19 cells were transiently transfected with a plasmid containing either the G or A allele for rs7747909 together with either hsa-miR-548s, hsa-miR-4480, or a miRNA negative control (NC). Renilla luciferase was measured and compared to firefly luciferase in three independent experiments. * p < 0.05.