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. 2016 Jan 15;11(1):e0146549. doi: 10.1371/journal.pone.0146549

Fig 5. Effects of transient and stable knockdown of LEDGF/p75 on ERp57 expression levels in PC3 cells.

Fig 5

LEDGF/p75 and ERp57 transcript and protein levels were assessed by qPCR and immunoblotting, respectively. A. Untransfected, parental PC3 cells with and without siRNA-induced transient depletion of LEDGF/p75; B. PC3-ledgf/p75 clones overexpressing LEDGF/p75 with and without siRNA-induced transient depletion of LEDGF/p75; C. PC3-viral-ledgf/p75 clones overexpressing LEDGF/p75 with and without siRNA-induced transient depletion of LEDGF/p75; D. PC3 cells with shRNA induced stable depletion of LEDGF/p75. Each graph represents the average of at least 3 independent experiments performed in triplicates (*P<0.05, **P <0.01). P values were determined in comparison to cells transfected with non-specific, scrambled control siRNAs (siSD or shSCR) using the Student’s t-test.