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. 2016 Jan 15;11(1):e0146549. doi: 10.1371/journal.pone.0146549

Fig 8. LEDGF/p75 binds to ERp57 promoter in ChIP assays.

Fig 8

A. Schematic diagram of ERp57 promoter. PCR primers targeted ERp57pr regions A (bp –498 to +1), B (bp –898 to – 490), C (bp – 890 to – 1298), D (bp – 1290 to – 1698), and E (bp – 1690 to – 2098). ChIP analysis of LEDGF/p75 binding to ERp57pr in PC3 (B) and U2OS (C) cells. Formaldehyde-fixed cells were precipitated with nonspecific IgG antibody or antibody specific for LEDGF/p75. PCR amplifications of immunoprecipitated DNA were carried out with primer sets specific for ERp57pr regions A to E. Primers for human β-actin or GAPDH were used to control for optimal enzymatic digestion of chromatin.