Fig 2. Progressive accumulation of cholesterol and GSLs in Npc1^nih/nih mice spleen.

(A) H&E staining of spleen sections of Npc1^+/+ and Npc1^nih/nih at 20, 40, 60 and 80 days of age. FC = follicle centre, PALS = peri-arteriolar lymphocyte sheath, WP = white pulp, RP = red pulp. Scale bar = 100 μm. (B) Levels of total cholesterol, ceramide and GSLs, glucosylceramide, lactosylceramide and globotriaosylceramide in spleen lysates of Npc1^+/+ (WT), Npc1^+/nih (HET) and Npc1^nih/nih (KO) mice at the age of 20–23, 40–43, 60–63 and 84–90 days. Data (n = 3–6, mean ± S.D.) were analysed using a two-way ANOVA: * P < 0.05, ** P < 0.01 and *** P < 0.001 from Npc1^+/+.