Fig 7. Gpnmb gene and protein expression are increased in macrophages exposed to U18666A.

RAW264.7 murine macrophages were exposed to 1, 5 and 10 μM of U18666A for 24 h. (A) Gpnmb mRNA levels and (B) Gpnmb protein levels. (C) Soluble Gpnmb assayed by ELISA of the cell culture medium. Data (n = 3 mean ± S.E.M.) were analysed using an unpaired t-test. * P < 0.05, ** P < 0.01 and *** P < 0.001 from DMSO control.