Fig. 3.

T5KO metabolic syndrome depends on hyperphagia. (A and B) Four-week-old WT and T5KO mice were given a high-fat diet for 8 weeks. (A) Fifteen-hour fasting blood glucose. (B) H&E stained pancreas (P, upper panel) and liver (L, lower panel). Arrows point to inflammatory infiltrates. (C) Mice on a regular diet were monitored for food intake. (D to G) Mice were subjected to food restriction and assayed for (D) body mass and (E) fat-pad mass. (F) Fifteen-hour fasting glucose. (G) Insulin sensitivity measured as described in Fig. 2E. Results in (A) and (B) are from a single experiment (n = 5 to 6) and representative of two independent experiments. Results in (C) are from a single experiment (n = 6) and representative of similar results with several groups of mice. (D) to (G) is from a single experiment (n = 10) and representative of two independent experiments. *, P < 0.05.