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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Curr Protoc Cell Biol. 2015 Dec 1;69:13.8.1–13.8.12. doi: 10.1002/0471143030.cb1308s69

Figure 1. Components of the assay.

Figure 1

The assay relies on three components: an FRB-tagged candidate vesicle binding protein, an FKBP-tagged molecular motor, and a means to specifically label the vesicles of interest. In the presence of linker drug, the FKBP and FRB domains heterodimerize. The vesicle is unaffected if the FRB-tagged candidate protein does not bind it (upper right). If the vesicle and the FRB-tagged candidate protein interact, then the presence of linker drug results in the recruitment of motors to the vesicle (lower right). Adapted from Jenkins et al., 2012.