Figure 1.

Deletion of five putative phosphatases hinders growth on eDNA as a source of phosphate.

Growth on eDNA was assessed using mid‐exponential phase bacteria, which were washed twice before putting in to the test conditions. The growth medium used was MOPS‐glucose supplemented with either (A) sheared salmon sperm DNA consisting of 0.5 mM phosphate or (B) no phosphate. The Δ5 phosphatases mutant is: ΔphoX ΔushA ΔcpdB ΔVCA0608 ΔVCA0545. Shown are two biological replicates assayed on the same day. The growth assay was performed two times, with a total of four biological replicates; each experiment exhibited the same results.