Video 7. Ablation of contractile ventral stress fibers does not affect the position of focal adhesions.
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DOI: 10.7554/eLife.06126.029
An example of a laser-ablated ventral stress fiber from a U2OS cell co-expressing mCherry-Actin and GFP-Zyxin. Five captures, every 1 s, were taken before the ablation, and retraction of the ventral stress fiber was followed for 10 x 1 s before bleaching the region below the focal adhesion. Recovery of mCherry-Actin signal was detected at the bleached area of the relaxed fiber. Display rate is 10 frames/s, and the total duration of the video is 9 min. Please note, that position of the focal adhesion (indicated by GFP-Zyxin) is not significantly affected by ablation of the associated stress fiber.