TABLE I.

Limits of detection (LOD), Limit of Quantification (LOQ), chi-squared, and R² values of bacterial detection (CFU/ml).

		LOD (CFU/ml)	LOD (bacteria/assay)	LOQ (CFU/ml)	LOQ (bacteria/assay)	chi2	R2
Buffer	Campylobacter	3.58 × 103	12	1.54 × 104	51	1.37 × 10−3	0.9993
	Listeria	5.80 × 103	19	6.65 × 104	222	1.89 × 10−3	0.99892
	Shigella	9.09 × 102	3	5.28 × 104	176	2.20 × 10−3	0.99834
	E. coli	2.33 × 103	8	4.01 × 104	134	7.30 × 10−3	0.99167
	Salmonella	6.72 × 103	22	1.34 × 105	447	2.55 × 10−3	0.99681
Ground water	Campylobacter	5.63 × 103	19	2.18 × 104	73	3.70 × 10−3	0.99638
	Listeria	9.19 × 103	31	2.70 × 104	90	5.11 × 10−3	0.99571
	Shigella	4.67 × 103	16	1.16 × 104	39	2.18 × 10−3	0.99812
	E. coli	1.14 × 103	4	3.69 × 103	12	4.90 × 10−3	0.99473
	Salmonella	4.58 × 103	15	1.40 × 104	47	2.70 × 10−3	0.99757
Tap water	Campylobacter	9.55 × 103	32	3.51 × 104	117	2.88 × 10−2	0.97294
	Listeria	4.51 × 103	15	1.52 × 104	51	5.61 × 10−3	0.99499
	Shigella	4.97 × 103	17	2.13 × 104	71	4.35 × 10−3	0.9952
	E. coli	3.57 × 103	12	1.39 × 104	46	2.73 × 10−3	0.99728
	Salmonella	9.43 × 102	3	3.8 × 103	13	5.01 × 10−3	0.99292
Multiplexed	Campylobacter	2.41 × 103	8	8.09 × 103	27	3.04 × 10−3	0.99588
	Listeria	5.13 × 103	17	2.06 × 104	69	1.36 × 10−3	0.9985
	Shigella	4.69 × 103	16	1.37 × 104	46	1.48 × 10−2	0.98556
	Salmonella	5.61 × 103	19	1.40 × 104	47	1.54 × 10−3	0.99834