Figure 1.
C. jejuni OMC extraction and fractionation. C. jejuni WT, Δppk1, and Δppk2 OMCs were fractionated into total lipids, proteins, and poly-and oligo-saccharides, as indicated in the flowchart and as described in detail in the Materials and Methods section. Fractions were normalized by weight before each extraction. The obtained fractions were analyzed for their contribution to invasion by and intracellular survival of C. jejuni in INT-407 cells.