Figure 4.

ErbB4 is necessary for Paneth cell development and intestinal epithelial integrity. A: Ileal enteroids from ErbB4^flox/flox (WT) and ErbB4^flox/flox;Vil-Cre (KO) mice were cultured in the presence or absence of 200 ng/mL of TNF for 4 days. RNA was prepared and analyzed by quantitative PCR for lysozyme expression (Rel. Lysz. Exp.) (reference gene: GAPDH). B: Bright-field microscopy showed representative morphology of WT and KO enteroids with or without TNF exposure. n = 3 independent experiments with cultures from three mice per genotype. Graphs show means ± SEM. ^∗P < 0.05, ^∗∗P < 0.01. Con, control.