Table 1.
Description of the Used Primary Antibodies for Flow Cytometric Analysis and Immunohistochemistry
| Antigen | Host | Clone number | Reactivity | Product | Distributor | Dilution rate | Tissue specimen |
|---|---|---|---|---|---|---|---|
| Flow cytometric analysis | |||||||
| Melanosome | Mouse, monoclonal | HMB45 | Human | M0634 | Dako | NA | NA |
| Melan-A | Mouse, monoclonal | A103 | Human | M7196 | Dako | NA | NA |
| c-Met | Rat, monoclonal | 11-8858-41 | Human | ab51067 | eBiosciences | NA | NA |
| IGF-1R | Mouse, monoclonal | 560934 | Human | 790-4346 | BD Biosciences Pharmigen | NA | NA |
| VEGF | Mouse, monoclonal | VG1 | Human | M7273 | Dako | NA | NA |
| VEGFR2 | Rabbit, monoclonal | 7D4-6 | Human | 359903 | BioLegend | NA | NA |
| Phospho-VEGF Receptor 2 | Rabbit, monoclonal | 19A10 | Human, Mouse | 2478 | Cell Signaling | NA | NA |
| Immunohistochemistry | |||||||
| Melanosome | Mouse, monoclonal | HMB45 | Human | M0634 | Dako | 1:50 | Paraffin embedded |
| Melan-A | Mouse, monoclonal | A103 | Human | M7196 | Dako | 1:50 | Paraffin embedded |
| S100 | Rabbit, polyclonal | none | Human, Mouse, Rat | Z0311 | Dako | 1:400 | Paraffin embedded |
| c-Met | Rabbit, monoclonal | EP1454Y | Human | ab51067 | Abcam | 1:100 | Paraffin embedded |
| Phospho-c-Met | Rabbit, monoclonal | D26 | Human, Mouse, Rat | 3077 | Cell Signaling | 1:150 | Paraffin embedded |
| IGF-1R | Rabbit, monoclonal | G11 | Human | 790-4346 | Ventana | 1:1.5 | Paraffin embedded |
| Phospho-IGF-1R | Rabbit, polyclonal | none | Human, Mouse | Sc-101703 | Santa Cruz | 1:500 | Paraffin embedded |
| CD31 | Rabbit, polyclonal | none | Human, Mouse | ab28364 | Abcam | 1:100 | Paraffin embedded |
| Laminin | Mouse, monoclonal | LAM-89 | Human | L8271 | Sigma-Aldrich | 1:1000 | Paraffin embedded |
| VEGF | Mouse, monoclonal | VG1 | Human | M7273 | Dako | 1:50 | Paraffin embedded |
| VEGF Receptor 2 | Rabbit, monoclonal | 55B11 | Human, Mouse | 2479 | Cell Signaling | 1:250 | Paraffin embedded |
| Phospho-VEGF Receptor 2 | Rabbit, monoclonal | 19A10 | Human, Mouse | 2478 | Cell Signaling | 1:100 | Paraffin embedded |
IGF-1R, insulin growth factor 1 receptor; NA, not applicable; VEGF, vascular endothelial growth factor.