Skip to main content
. 2014 Oct;184(10):2611–2617. doi: 10.1016/j.ajpath.2014.06.009

Figure 4.

Figure 4

Up-regulated CTGF/CCN2 expression in TGF-βIIR knockout (TRII-KO) livers at 8 weeks of carbon tetrachloride treatment. A: Real-time PCR analysis of CTGF/CCN2 mRNA level. B: Tissue distribution of CTGF/CCN2 (CTGF, red) and DAPI for cell nuclei (blue) in the mutant liver by immunofluorescence staining. C: Cellular distribution of CTGF/CCN2 (CTGF) in the mutant liver. Double immunofluorescence staining for CTGF/CCN2 (red) and DAPI for cell nuclei (blue), and triple staining for CTGF/CCN2 (red), hepatocyte marker albumin (green), and DAPI (blue) in the same image. mRNA expression level is shown relative to the control value of 1. Error bars represent SD. n = 5 (mutant mice); n = 8 (control). ∗∗P < 0.01. Scale bars: 100 μm (B); 25 μm (C).