Figure 1.
WGA and SNP array profiling on LNCaP populations of one and five- cells are reproducible and accurate. A: Representative images of LNCaP from bulk gDNA (DNA extracted from 2 × 106 cells), WGA2 (GenomePlex Complete Whole Genome Amplification kit)-amplified from 10 ng DNA, and Picoplex WGA amplified five- and one-cell aliquots of LNCaP. B: Schematic presentation of chromosome 10 (82,394,009 to 106,849,461 bp, red box) showing three deletions of 4.46 Mb, 0.71 Mb, and 1.94 Mb in size. The Hudson alpha track from University of California Santa Cruz genome browser was aligned to the corresponding regions. SNP, single nucleotide polymorphism; WGA, whole-genome amplification.