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. 2014 Oct;184(10):2687–2698. doi: 10.1016/j.ajpath.2014.06.014

Figure 10.

Figure 10

TSP1 deficiency attenuates TGF-β1 signaling. A and C: Representative photomicrographs of formalin-fixed, paraffin-embedded kidney sections of wild-type control (32 weeks) and creatinine-matched Col4a3 KO and Col4a3;Tsp1 DKO mice. A: Sections were labeled with antibodies to TSP1 (red) and counterstained with methylene green. In the fibrotic Col4a3 KO kidneys, TSP1 was localized predominantly in tubular epithelial cells, blood vessels, and interstitial cells. B: The percentage of atrophic tubules was significantly greater in kidneys of Col4a3 KO mice than in Col4a3;Tsp1 DKO mice. C: Sections were labeled with antibodies to phosphorylated SMADs 2 and 3 (p-SMAD2/3), indicative of active signaling in response to TGF-β1 (red) and counterstained with methylene green. D: Numbers of p-SMAD2/3+ nuclei per visual field. SMAD2/3 signaling was significantly enhanced in tubular and interstitial compartments of kidneys of Col4a3 KO mice, compared with creatinine-matched Col4a3;Tsp1 DKO kidneys. Data are expressed as means ± SEM. *P < 0.05. Original magnification, ×40.