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. 2014 Oct;184(10):2687–2698. doi: 10.1016/j.ajpath.2014.06.014

Figure 11.

Figure 11

TSP1 deficiency causes an altered response to latent TGF-β1. A: Fibroblast proliferation in response to active and latent TGF-β1. Primary fibroblasts isolated from kidneys of wild-type control and TSP1-deficient mice (Tsp1 KO) were exposed for 24 hours to 5 ng/mL of either latent TGF-β1 or active TGF-β1. Relative proliferation was assessed by a WST assay. TSP1 deficiency significantly impaired the proliferative response to latent TGF-β1, but not to active TGF-β1. B: Type III collagen expression in response to active and latent TGF-β1. Wild-type control and Tsp1 KO renal fibroblasts were exposed for 24 hours to 5 ng/mL of either latent TGF-β1 or active TGF-β1. Type III procollagen expression was assessed by quantitative real-time PCR. TSP1 deficiency significantly decreased collagen III expression in response to latent TGF-β1. Data are expressed as means ± SEM. *P < 0.05. Col3, collagen III; rel., relative.