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. 2014 Oct;184(10):2687–2698. doi: 10.1016/j.ajpath.2014.06.014

Figure 6.

Figure 6

Interstitial accumulation of Th1 and Th2 cells. A and C: Representative microphotographs of formalin-fixed, paraffin-embedded kidney sections of wild-type control (32 weeks) and creatinine-matched Col4a3 KO and Col4a3;Tsp1 DKO mice. Sections were labeled with antibodies against C-C chemokine receptor type 5 (CCR5, a marker of Th1 cells) (A) or with G-protein coupled receptor 44 (GPR44, a marker of Th2 cells) (C) and counterstained with methylene green. B and D: Numbers of CCR5+ T cells (B) and GPR44+ T cells (D) per visual field. Col4a3 KO mice exhibited interstitial accumulation of GPR44+ Th2 cells; in creatinine-matched Col4a3;Tsp1 DKO mice, TSP1 deficiency was associated with a Th1 switch and accumulation of CCR5+ Th1 cells. Data are expressed as means ± SEM. *P < 0.05. Original magnification, ×40.