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. 2014 Oct;184(10):2687–2698. doi: 10.1016/j.ajpath.2014.06.014

Figure 7.

Figure 7

Accumulation of Th17 and T-regulatory cells. A and C: Representative microphotographs of formalin-fixed, paraffin-embedded kidney sections of wild-type control (32 weeks) and creatinine-matched Col4a3 KO and Col4a3;Tsp1 DKO mice. Sections were labeled with antibodies against RAR-related orphan receptor-γ (ROR-γ, a marker of Th17 cells) (A) or forkhead box protein P3 (FOXP3, a marker of T-regulatory cells) (C) and counterstained with methylene green. B and D: Numbers of ROR-γ+ (B) and FOXP3+ (D) T cells per visual field. TSP1 deficiency in creatinine-matched Col4a3;Tsp1 DKO mice was associated with loss, relative to Col4a3 KO mice, of Th17 cells (ROR-γ+) and T-regulatory cells (FOXP3+). Data are expressed as means ± SEM. *P < 0.05. Original magnification, ×40.