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. 2014 Oct;184(10):2687–2698. doi: 10.1016/j.ajpath.2014.06.014

Figure 8.

Figure 8

A: Representative photomicrographs of kidney sections of wild-type control (32 weeks) and creatinine-matched Col4a3 KO and Col4a3;Tsp1 DKO mice. Sections were immunolabeled with CD31 (red), a marker of vasculature; the basal membrane was stained with an antibody to collagen IV (green) that recognizes collagen IV α1/2 chains and thereby also detects collagen IV in Col4a3 KO and Col4a3;Tsp1 DKO mice. Nuclei were counterstained with DAPI. B: Numbers of microvascular intersections per visual field. TSP1 deficiency in Col4a3;Tsp1 DKO mice had no additional effect on microvascular density, compared with Col4a3 KO mice. Data are expressed as means ± SEM. Original magnification, ×60. Col4, collagen IV.