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Phospho-H2AX (γ-H2AX) expression and oxidative stress in D1 cells exposed to As+3. D1 cells were treated with 50 or 500 nM As+3. Phospho-H2AX expression levels were measured by flow cytometry (A), represented by Mean Channel Fluorescence or Western blot (B). C, Mean Channel Fluorescence of DHE. D, HO-1 RNA levels were measured by qPCR on cDNA samples from As+3-treated D1 cells. *Significantly different compared with control (P < .05). Results are means ± SD.
