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. 2016 Jan 16;17:63. doi: 10.1186/s12864-016-2373-3

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© McGrath et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Schematic drawing of the lobster Homarus americanus, indicating tissue types collected for this study. Central nervous system tissue samples include either abdominal ganglia (abdominal nerve cord) or the supraesophogeal ganglion (brain). All heart tissue samples include the heart muscle with attached cardiac ganglion and neurosecretory pericardial organ. Muscle tissue samples are a section of the abdominal muscle. Adapted with permission from John Wiley and Sons, from Skiebe 1999. b Principal component analysis of transcripts across three tissue types (heart, muscle, and central nervous system tissue) and two treatment levels (hormone treated and untreated). This 2D representation of the first two principal components of these 10 samples demonstrates a greater effect of tissue type than treatment, and also a greater effect of tissue type than variation across individuals. Component 1 explains 87.84 % of the variance (SD = 2.96); component 2 explains 4.08 % of the variance (SD = 0.64)