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. 2016 Jan 16;16:23. doi: 10.1186/s12885-016-2055-1

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© Dutta et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Autophagy Flux Measurement. HT-29 cells were treated with 2c for different time intervals (12, 24, 48 h) and one set with Rapamycin (positive control) for 24 h only. Autophagy was measured by staining autophagosomes and autophagic compartments with the fluorescent probe Cyto-ID® Green. a Control cells (b) positive Control and 2c treated HT-29 cells of (c) 12 h, (d) 24 h, (e) 48 h were stained and analyzed in the green (FL1) channel of the FACS Caliber flow cytometer. f The graphical representation of autophagosome formation shown increasing percentage of autophagosomes with respect to control cells according to the different time periods. This indicates increase in autophagy flux formation in a time dependent manner. The figures are representative profile of at least three experiments