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. 2016 Jan 16;16:23. doi: 10.1186/s12885-016-2055-1

Fig. 5.

Fig. 5

Conversion of LC3A to LC3B and densitometric analysis. a Control and 2c treated (14.9 μM; 0–48 h) whole cell lysates of HT-29 cells were prepared and analysed by western blotting to study the conversion of LC3A to LC3B. β-actin was used as loading control. The figure is a representative profile of at least three experiments. b Bands obtained from western blotting were quantified by densitometric analysis and expression level of LC3 protein was determined. Histograms represent LC3 protein expression normalized to β-actin (***p <0.001)