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. 2016 Jan 16;16:23. doi: 10.1186/s12885-016-2055-1

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© Dutta et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effect of autophagy inhibitor 3-MA in 2c induced cytotoxicity. HT-29 cells (2.5 × 10⁴/100 μl of RPMI 1640 medium / well) were pre-treated with autophagy inhibitor, 3-MA (10 mM) for 4 h followed by 2c treatment for 48 h. Cell viability was measured by MTT assay. Histograms represent percentage cell viability (Mean ± SEM) obtained at the IC₅₀ concentration of 2c (14.9 μM) and has been derived from at least three experiments in duplicate (***p <0.001 compared to only 2c treated cells)