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. 2016 Jan 16;16:23. doi: 10.1186/s12885-016-2055-1

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© Dutta et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — 2c-induced expression of Beclin1 and LC3B mRNA. 2c treated and untreated HT-29 cells were subjected to RNA isolation and the expression of mRNA was analysed. Beclin1 and LC3B expression in HT-29 cells treated with or without 2c were measured by qRT-PCR and normalized to GAPDH expression. Data are expressed as fold change relative to untreated cells showing induction of autophagy in cells treated with 2c for different time intervals (12, 24 and 48 h)