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Protein kinase A expression and activity. mRNA (A) and protein (B) extracts were prepared from vegetative cells and cells at different time points of development. Northern blots (A) were probed to visualise PKA-C mRNA, and Western blots (B) were probed with anti PKA-C antibody. (C) PKA enzyme activity, measured in vegetative cells at a density of 2 × 105 cells/ml growing on bacteria. Data shown represent mean ± SE from 3 independent experiments.
