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Aggregation of gdt2- cells in the presence of amino acids and folate. Cells were harvested from axenic culture at exponential growth phase, washed and plated at a density of 2 × 105 cells/cm2 in buffer containing either amino acids (A) or 1 mM folate (B). In the presence of amino acids gdt2- mutant cells aggregate 4–5 hours earlier than wild type, while in the presence of folate they aggregate only 1 hour earlier than wild type, as they do in unsupplemented buffer.
