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. 2004 Jun 29;5:38. doi: 10.1186/1471-2164-5-38

Figure 7.

Figure 7

Overshining correction. The same spotting pattern at three different densities: 375 μm, 750 μm, and 1500 μm inter-spot distance. Comparison of the 375 μm pattern with the 1500 μm one shows a larger dispersion than the 750 μm/1500 μm comparison: this is the overshining effect. The red points are the spots detected as significantly affected by overshining in the 375 μm density. Applying a correction computed via the fit reduces the dispersion. Black lines indicate background levels.