Figure 5. Increase of Bnr1 polymerization rate in simulations reproduces phenotype of smy1Δ cells.

(A) Projection snapshots taken at 80 s (as in Figure 2C) as a function of increasing Bnr1 polymerization rate show an increase in cable curvature, see Movie S4. (B) Experimental images of phalloidin-labeled actin cables reprinted from [Chesarone-Cataldo et al. 2011] with permission from Elsevier. The smy1Δ cells show long and more curved actin cables compared to wild type. (C) Number of cable bends and turns as a function of Bnr1 polymerization rate (manual counting using simulation projection snapshots). Data: 15 time points starting at 50 s, separated by 5 s from 3 independent runs (same for D, E). Error bar is one standard deviation. (D) Average curvature increases with increasing polymerization rate. (E) Average radial distance (measured from the axis that runs through the centers of mother and bud) of actin filaments as a function of Bnr1 polymerization rate. The average radial distance increases with increasing Bnr1 polymerization rate below the neck (z = 1 µm) and at the mother center (z = 0 µm). No significant change for cross-section at the bud center (z = 3.3 µm).